Microbiological tests

The purpose of microbiological tests is to monitor contamination during the entire process of cosmetics production, starting from the acquisition of raw materials and production hygiene and ending with the finished product. These tests verify the degree of microbiological purity of cosmetics and thus confirm their safety, in accordance with the acceptable levels set out in PN-EN ISO 17516 Cosmetics – Microbiology – Microbiological limits.

Microbiological purity – quantitative and qualitative testing

We carry out accredited qualitative tests, including identification of the presence of typical pathogens and quantitative tests of the total number of microorganisms, according to ISO methods:

  • number of aerobic mesophilic bacteria according to PN EN ISO 22149;
  • number of yeasts and moulds according to EN ISO 16212;
  • presence of Staphylococcus aureus according to EN ISO 22718;
  • presence of Pseudomonas aeruginosa according to EN ISO 22717;
  • presence of Escherichia coli according to EN ISO 21150;
  • presence of Candida albicans according to PN EN ISO 18416.

Preservation test – challenge test

The test confirms the antimicrobial properties of preservatives, as well as their effectiveness in the formulation system. The preservation test confirms that the applied preservatives protect the cosmetic product against microbial growth and prevent the development of secondary infections during the intended use.

Stress tests are performed according to the methodology of:

  • PN EN ISO 11930 standard;
  • Polish or European Pharmacopoeia;
  • Schülke&Mayr so-called Koko test;
  • Adapted individually to the customer’s requirements – in particular, targeting strains obtained from raw materials or directly from the production environment in the production area of a given plant.

Confirmation of antimicrobial activity

Tests are performed to confirm or exclude the antimicrobial activity of the product against selected test strains obtained from recognised collections or swabs from the skin surface of probands. Testing is performed on the basis of an internal procedure using the method:

  • suspension (determining the degree of microbial reduction);
  • disc (determining the zone of inhibition of microbial growth).



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